Journal: PLoS ONE

Article Title: Study of the Integrated Immune Response Induced by an Inactivated EV71 Vaccine

doi: 10.1371/journal.pone.0054451

Figure Lengend Snippet: A global view of gene modulation in the PBMCs of vaccinated infants (Vac, n = 20), 28 days after the vaccine booster, compared to the placebo cohort (Pla, n = 10) was determined by microarray analysis. The genes were classified according to their function. A heat map representation (a) and principal component analysis (PCA) (b) generated using the significantly modulated genes from at least one comparison versus day 0 are shown. (a) The color scale shows the significantly modulated genes from up- (dark red) to down-regulated (dark blue) to the right of each image. The values are shown on a log 2 scale. (b) The Y value is the log 2 -fold-changes in gene expression of the vaccinated group vs. the placebo group. The up-regulated changes are above the baseline (Y = 0, the same with placebo group), and the down-regulated changes are below the baseline. The bars represent the maximum and minimum. The mean of the fold-changes in gene expression with a 95% CI is shown as the rectangle. The line in the rectangle represents the mean of these fold changes in gene expression. The number of genes that changed after immunization is shown beside the rectangles on the image.

Article Snippet: After hybridization, the microarrays were scanned in the Agilent Microarray Scanner (Cat# G2565CA, Agilent, CA, USA), and the raw data were obtained by Feature Extraction Software 10.7 (Agilent, CA, USA) and normalized using the Quantile algorithm in Gene Spring 11.0 (Agilent, CA, USA).

Techniques: Microarray, Generated, Expressing

Journal: PLoS ONE

Article Title: Study of the Integrated Immune Response Induced by an Inactivated EV71 Vaccine

doi: 10.1371/journal.pone.0054451

Figure Lengend Snippet: Measure of serum proinflammatory cytokines (a) and the modulation of the expression of genes associated with the inflammatory response (b). (a) Levels of serum proinflammatory cytokines in vaccinated and placebo subjects. All cytokine (IL-1b, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL12p70, TNF-α and IFN-γ) levels were measured with an R&D Systems assay kits according to the manufacturer protocol. (b) The genes modulated and associated with the inflammatory response in vaccinated group (Vac) and placebo group (Pla) were classified and fallen under different functional categories: (1) ILs group: IL1A, IL1B, IL2RA, NFKBIZ (activator of IL-6 expression), IL8, IL17C, IL26 and IL32 in the IL group; (2) TNFs group: TNFAIP2 and TNFAIP6; (3) IFNs group: IFNA10 and OAS2 (activator of IFN-γ); (4) chemokines group: CCL3, CCL4, CCL15, CCL23, CCL26, CXCL1, CXCL3 and CXCR7. The levels of gene expression were measured by microarray analysis as described in the Methods. Data are shown in log 2 scale.

Article Snippet: After hybridization, the microarrays were scanned in the Agilent Microarray Scanner (Cat# G2565CA, Agilent, CA, USA), and the raw data were obtained by Feature Extraction Software 10.7 (Agilent, CA, USA) and normalized using the Quantile algorithm in Gene Spring 11.0 (Agilent, CA, USA).

Techniques: Expressing, Functional Assay, Microarray

Journal: Data in Brief

Article Title: Circulating miRNome profiling data in Behçet's syndrome

doi: 10.1016/j.dib.2021.107435

Figure Lengend Snippet: Demographics of the patients and controls included in the microarray screening cohort.

Article Snippet: Description of data collection , Data obtained by performing miRNA Agilent Microarray hybridization experiments, followed by data extraction and analysis using available dedivcated scripts for data pre-processing and expression analysis. Expression data were compared between patients and healthy controls..

Techniques: Microarray

Journal: Data in Brief

Article Title: Circulating miRNome profiling data in Behçet's syndrome

doi: 10.1016/j.dib.2021.107435

Figure Lengend Snippet: DE miRNAs identified by microarray analysis in the screening phase.

Article Snippet: Description of data collection , Data obtained by performing miRNA Agilent Microarray hybridization experiments, followed by data extraction and analysis using available dedivcated scripts for data pre-processing and expression analysis. Expression data were compared between patients and healthy controls..

Techniques: Microarray, Sequencing

Journal: Data in Brief

Article Title: Circulating miRNome profiling data in Behçet's syndrome

doi: 10.1016/j.dib.2021.107435

Figure Lengend Snippet: GEO microarray data description.

Article Snippet: Description of data collection , Data obtained by performing miRNA Agilent Microarray hybridization experiments, followed by data extraction and analysis using available dedivcated scripts for data pre-processing and expression analysis. Expression data were compared between patients and healthy controls..

Techniques: Microarray

Journal: Data in Brief

Article Title: Circulating miRNome profiling data in Behçet's syndrome

doi: 10.1016/j.dib.2021.107435

Figure Lengend Snippet:

Article Snippet: Description of data collection , Data obtained by performing miRNA Agilent Microarray hybridization experiments, followed by data extraction and analysis using available dedivcated scripts for data pre-processing and expression analysis. Expression data were compared between patients and healthy controls..

Techniques: Microarray, Hybridization, Labeling, Expressing

Journal: Integrative zoology

Article Title: cba-miR-222-3p involved in photoperiod-induced apoptosis in testes of striped hamsters by targeting TRAF7.

doi: 10.1111/1749-4877.12918

Figure Lengend Snippet: Figure 3 Validation of cba-miR-222-3p targeting TRAF7 and TRAF7 expression in the testes of striped hamsters. (a) Sequences and peak maps of cba-miR-222-3p, TRAF7-WT, and TRAF7-MT. (b) Relative luciferase activity detected by Dual-Luciferase Reporter Assay. (c) Immunohistochemistry (IHC, tissue microarray [TMA]) of TRAF7 in testes. (d) Integrated density of TRAF7 detected by IHC (TMA; n = 4). (e) Protein expression levels of TRAF7 in the testes detected by western blot (n = 4). (f) Pearson correlation analysis of cba-miR-222-3p and TRAF7. LD, long daylength; MD, moderate daylength; SD, short daylength; ∗, P < 0.05; ∗∗, P < 0.01.

Article Snippet: After performing electrophoresis, the proteins were transferred to PVDF membranes, which were then incubated with TRAF7 antibodies (11780-1-AP, Proteintech, China, RRID:AB_2877793) at a 1:1000 dilution and β-actin antibodies (20536-1-AP, Proteintech, China, RRID:AB_10700003) at a 1:1000 dilution, and subsequently incubated with IRDye 800 CW goat anti-rabbit secondary antibodies (31 460, Thermo Fisher, USA).

Techniques: Biomarker Discovery, Expressing, Luciferase, Activity Assay, Reporter Assay, Immunohistochemistry, Microarray, Western Blot

Journal: Integrative zoology

Article Title: cba-miR-222-3p involved in photoperiod-induced apoptosis in testes of striped hamsters by targeting TRAF7.

doi: 10.1111/1749-4877.12918

Figure Lengend Snippet: Figure 4 Expression of cba-miR-222-3p and TRAF7 and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) analysis after in vivo injection in the testes of striped hamsters. (a) Schematic diagram of in vivo injection experiment of miRNA mimics. (b) Fluorescence in situ hybridization (FISH, tissue microarray [TMA]) of cba-miR-222-3p in testes after in vivo injection. (c) Fluorescence intensity of cba-miR-222-3p detected by FISH (TMA; n = 4). (d) Immunohistochemistry (IHC, TMA) of TRAF7 in testes after in vivo injection. (e) Integrated density of TRAF7 detected by IHC (TMA; n = 4). (f) TUNEL (TMA) staining of the testes after in vivo injection. (g) The proportion of TUNEL (TMA) staining with apoptotic activity in the testes (n = 4). (h) Relative expression of MEKK3 (n = 6). (i) Relative expression of p38 (n = 6). (j) Relative expression of p53 (n = 6). AG, agomir injection; NC, agomir negative control injection; DAPI, 4′6′-diamidino-2-phenylindole. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001.

Article Snippet: After performing electrophoresis, the proteins were transferred to PVDF membranes, which were then incubated with TRAF7 antibodies (11780-1-AP, Proteintech, China, RRID:AB_2877793) at a 1:1000 dilution and β-actin antibodies (20536-1-AP, Proteintech, China, RRID:AB_10700003) at a 1:1000 dilution, and subsequently incubated with IRDye 800 CW goat anti-rabbit secondary antibodies (31 460, Thermo Fisher, USA).

Techniques: Expressing, TUNEL Assay, In Vivo, Injection, Fluorescence, In Situ Hybridization, Microarray, Immunohistochemistry, Staining, Activity Assay, Negative Control